USING WING-SPECIFIC DRIVERS TO ASSESS THE IMPORTANCE OF CNV’S ON TISSUE DEVELOPMENT
Open Access
Author:
Yennawar, Sneha Hemant
Millennium Scholars Program:
Biochemistry and Molecular Biology (B M B)
Degree:
Bachelor of Science
Document Type:
Thesis
Thesis Supervisor:
Dr. Santhosh Girirajan, Thesis Supervisor
Keywords:
Drosophila RNAi Neurodevelopmental Disorders
Abstract:
Genes that have been established as important for nervous tissue development could have further implications in general tissue development. In order to assess this possibility among gene regions with established relationships to neurodevelopmental disorders, this study sought to test individual genes from these regions within the wing pouch tissue of Drosophila melanogaster. As part of this high throughput study, 10 such regions encompassing 58 genes were analyzed, and lines of the genes totaled 117. While many tests assaying the success and efficiency of the RNAi knockdown should be conducted on these lines, the results of this study indicate that there are 16 genes that definitely contribute to the development of non-nervous tissue. This number likely would increase once qPCR would reveal the knockdown success (or lack thereof) in genes with discordant lines.
